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MRC Prion Unit and Institute of Prion diseases

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Jan Bieschke

Structure and dynamics of prions and their ligand interactions

Jan Bieschke

J.Bieschke@prion.ucl.ac.uk

Tel: 020 7679 5128
Courtauld Building, Room 101B

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Research Synopsis

We aim to understand the central problem in the prion mechanism; what is the change in shape that distinguishes normal prion protein, PrPCÌý, from its rogue form, PrPScÌý, and how does it come about? Specifically, we are asking what the structural causes are for becoming a prion, what the common drivers are for their replication,Ìý and whether the same principles underlie other diseases, both inside the central nervous system and in the rest of the body.

We combine biophysical techniques with nanoscopic imaging to study the structure, folding and dynamics of prions, both in isolation and with likely binding partners, in order to develop new strategies against prion diseases.ÌýWe have developed nanoscopic imaging to observe amyloid structures over extended times through transient amyloid binding (TAB) of dye molecules.

A population of prions can have individual prion particles with different folds, which template different fibril structures during elongation. These individual prion particles form a ‘quasi-species’, where individual particles compete for substrate and where the replication environment shapes the replication ‘fitness’ of each prion particle. Fibril structure can ‘mutate’ in mid-growth as shown in the movie.

Prions therefore recapitulate the basic molecular principles of Darwinian evolution in a system, which does not contain genetic information coded by DNA or RNA. (Sun et al. ACS Nano 2023)

Fig 1
Left: Cluster of PrP fibrils grown from a single seed. Different polarization signatures in TAB imaging reflect differences in fibril structures. Right: Different fibril types elongate by distinct mechanisms (image taken from: Sun, ACS nano 2023).
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Growth of a single PrP fibril, which changes conformation in mid growth causing a switch from straight to helical morphology.Ìý

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Selected Publications


Y Sun, K Jack, T Ercolani, D Sangar, L Hosszu, J Collinge, J Bieschke ACS nano 2023


LLP Hosszu, D Sangar, M Batchelor, E Risse, AM Hounslow, J Collinge, J Bieschke.
Journal of Molecular Biology 2023


GR Nahass, Y Sun, Y Xu, M Batchelor, M Reilly, I Benilova, N Kedia, ... ,J Bieschke.
Journal of molecular biology 2021


LLP Hosszu, R Conners, D Sangar, M Batchelor, EB Sawyer, S Fisher, ...
Communications Biology 2020

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